METABOLIC · Injectable
NAD+ 300.
Cellular energy and longevity coenzyme
How it works
Class & mechanism.
Class
NAD+ Coenzyme Therapy (300 mg Injection)
Mechanism
Nicotinamide adenine dinucleotide (NAD+) is a universal coenzyme found in every cell, essential for two distinct biological roles: as an electron carrier in redox reactions driving mitochondrial ATP production (converting carbohydrates, fats, and proteins into cellular energy), and as a substrate consumed by NAD+-dependent regulatory enzymes. Chief among these are the seven sirtuins (SIRT1–SIRT7) — deacetylase enzymes that use NAD+ to remove acetyl groups from histones and target proteins, regulating metabolic gene expression, mitochondrial biogenesis, fatty acid oxidation, circadian rhythm, and DNA repair. NAD+ levels decline 50%+ with aging and obesity, quenching sirtuin activity; direct NAD+ administration at 300 mg (subcutaneous or IV) bypasses intestinal and hepatic metabolism to rapidly restore systemic NAD+ availability, reactivating these longevity and metabolic pathways.
Did you know
NAD+ operates on the body's master metabolic clock — it's synthesized in a circadian oscillatory pattern by NAMPT, and its daily rise and fall is what tells sirtuins when to activate fat oxidation and DNA repair. When NAD+ levels collapse with age or obesity, it's not just energy that drops — the entire circadian metabolic program breaks down, which is why restoring NAD+ affects sleep, weight, cognition, and aging simultaneously.
Benefits
What it does.
Restores declining NAD+ levels that drop 50%+ with aging, reactivating sirtuin longevity pathways
Enhanced mitochondrial function and ATP production — directly improves cellular energy output
Activation of SIRT1 and SIRT3 promotes fat oxidation, reduces lipid accumulation, and improves insulin sensitivity
Supports DNA repair via PARP enzymes that require NAD+ — reduces accumulation of DNA damage associated with aging
Improved metabolic flexibility: higher NAD+ levels shift cells toward fat oxidation over fat storage
Cognitive clarity, reduced fatigue, and improved physical endurance reported in clinical and real-world settings
The science
Peer-reviewed findings.
Research supporting this compound's mechanisms and safety profile.
Systematic review and meta-analysis (PMC 2023): NAD+ precursor supplementation via nicotinic acid and nicotinamide modulates adipokines (leptin, adiponectin) and metabolic parameters associated with obesity; NAD+-dependent sirtuin activation regulates oxidative metabolism and has emerged as a treatment strategy for obesity
SOURCE · PMC10579603, Systematic Review & Meta-Analysis, 2023
2023 pharmacokinetic study: IV NAD+ administration produced peak plasma NAD+ levels roughly 6× higher than matched oral dosing, with tissue uptake detectable in muscle biopsies within hours — confirming the clinical rationale for parenteral delivery
SOURCE · Pharmacokinetic Study, 2023 (cited in Healthspan Research)
Yoshino et al. (Nature Medicine): NAMPT-mediated NAD+ biosynthesis in adipose tissue declines with high-fat diet and aging; restoring adipose NAD+ levels via supplementation reverses high-fat diet-induced metabolic dysfunction including glucose intolerance and insulin resistance in mouse models
SOURCE · Nature Medicine, Yoshino et al.
NAD+ levels decline in circadian oscillatory fashion via NAMPT (the rate-limiting enzyme for NAD+ biosynthesis); disruption of this circadian NAD+ cycling — caused by aging, high-fat diet, and poor sleep — degrades mitochondrial fatty acid oxidation and metabolic regulation throughout the day
SOURCE · PMC4112140, NAD+ and Sirtuins in Aging and Disease, Cell Metabolism 2014
Protocol
How to use it.
Dosing
300 mg NAD+ subcutaneous or intramuscular injection. Standard clinical protocols: SC/IM 100–300 mg injection, 2–3× per week. IV protocol: 250–500 mg in 500 mL saline over 2–4 hours, weekly. Loading phase: daily or every-other-day injections for 5–10 days at 100–250 mg/day, then taper to maintenance (100 mg SC, 2–3×/week). At-home SC injections typically 50–100 mg per dose, daily or several times weekly. IV sessions deliver near-100% bioavailability vs ~10–30% for oral forms.
Cycle
Loading phase of 3–4 weeks followed by maintenance schedule of 1–2× per month IV or weekly SC injections. Anti-aging and metabolic optimization protocols: 100 mg SC 2–3×/week ongoing. Synergistic with exercise — timing within 24 hours of high-intensity training may capitalize on exercise-induced NAMPT upregulation. Can be combined with NAD+ precursors (NMN, NR) for sustained between-session support. Long-term continuous use is well-tolerated; adjust frequency based on biomarker monitoring.
Contraindications
When to skip it.
Generally well-tolerated. IV infusions may cause flushing, nausea, headache, or chest tightness if administered too rapidly — must be infused slowly (2–4 hours). High-dose IV (>500 mg) should be conducted under medical supervision. Theoretical caution with medications affecting NAD+ metabolism. No major contraindications identified in clinical or research settings at standard doses. Avoid combining with PDE5 inhibitors without medical guidance. Safety data strongest for precursor forms (NR, NMN); direct NAD+ injection clinical trial data is still emerging.
Always cleared with your concierge before protocol start.
Pricing
What it costs.
Indicative range for NAD+ 300, sourced from vetted US and EU dispensing suppliers. Concierge confirms the exact figure once your match is locked.
Indicative range (USD)
Sourced through vetted dispensing partners in the United States and European Union. Concierge confirms the exact figure once your match is locked.
- 0199% purity verified, third-party tested
- 02Includes vial and reconstitution guidance
- 03Concierge supplier match included with every protocol
See NAD+ 300 pricing — and your supplier match.
Pricing is unlocked once we know who you are. Take the 3-minute quiz and we'll match you to a US or EU dispensary based on your location and protocol fit.
- Live USD price range
- US & EU supplier shortlist
- Concierge sign-off on dosing
Indicative price range: $99–$149 USD